High constriction ratio continuous insulator based dielectrophoretic particle sorting
Name
900636680-MIT.pdf
Description
Full printable version
Size
7.22 MB
Format
Adobe PDF
Checksum (MD5)
07a753aecf29f42e63780d643e22f187
Author(s)
Wang, Qianru, Ph. D. Massachusetts Institute of Technology
Advisor(s)
Cullen R. Buie.
Date Issued
2014
Publisher
Massachusetts Institute of Technology
Abstract
Low frequency insulator based dielectrophoresis (iDEP) is a promising technique to study cell surface dielectric properties. To date, iDEP has been exploited to distinguish, characterize, and manipulate particles and bacteria based on their size and general cell phenotype (e.g. gram positive vs. gram negative). However, separation of bacteria with diverse surface phenotypes but similar sizes sets a much higher demand on separation sensitivity, necessitating improvement in channel structure design in order to increase the electrical field gradient. In this work, a three dimensional insulator based dielectrophoresis (3DiDEP) microdevice is designed to achieve continuous particle sorting based on their size and, more importantly, on their surface dielectric properties. A 3D constriction is fabricated inside Poly (methyl methacrylate) (PMMA) channels using a micromilling technique. By controlling the channel geometry at the 3D constriction area, a nonuniform electric field with a large intensity gradient perpendicular to the local particle flow direction results in transverse particle deflection, driving particles into different outlet streams. With both simulation and experiments, we show that a diverse array of particles can be distinguished by 3DiDEP. This 3DiDEP sorter can be used in multiple applications in which the surface properties of cells or particles are of special interest.
Description
Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2014.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 75-80).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
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