MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum
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Published version
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Author(s)
Xiong, Aoli
Kaushal, Simran
Tay, Ian Junjie
Engelward, Bevin P
Han, Jongyoon
Preiser, Peter R
Date Issued
2021
Journal
STAR Protocols
Publisher
Elsevier BV
Citation
Xiong, Aoli, Kaushal, Simran, Tay, Ian Junjie, Engelward, Bevin P, Han, Jongyoon et al. 2021. "MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum." STAR Protocols, 2 (3).
Version
Final published version
Abstract
Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).
MIT Department
Singapore-MIT Alliance in Research and Technology (SMART)
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/J.XPRO.2021.100797
