Graphene Oxide Nanosheets Modified with Single-Domain Antibodies for Rapid and Efficient Capture of Cells
Name
Ploegh_Graphene oxide.pdf
Size
593.7 KB
Format
Adobe PDF
Checksum (MD5)
d954ada9392bd848137198eff991ecaa
Author(s)
Chen, Guan-Yu
Theile, Christopher S.
Duarte, Joao N.
Maruyama, Takeshi
Rashidfarrokh, Ali
Bardhan, Neelkanth Manoj
Kumar, Priyank Vijaya
Belcher, Angela M
Ploegh, Hidde
Li, Zeyang,S.M.Massachusetts Institute of Technology.
Date Issued
October 2015
Journal
Chemistry - A European Journal
Publisher
Wiley Blackwell
Citation
Chen, Guan-Yu et al. “Graphene Oxide Nanosheets Modified with Single-Domain Antibodies for Rapid and Efficient Capture of Cells.” Chemistry - A European Journal 21.48 (2015): 17178–17183.
Version
Author's final manuscript
Abstract
Peripheral blood can provide valuable information on an individual’s immune status. Cell-based assays typically target leukocytes and their products. Characterization of leukocytes from whole blood requires their separation from the far more numerous red blood cells.1 Current methods to classify leukocytes, such as recovery on antibody-coated beads or fluorescence-activated cell sorting require long sample preparation times and relatively large sample volumes.2 A simple method that enables the characterization of cells from a small peripheral whole blood sample could overcome limitations of current analytical techniques. We describe the development of a simple graphene oxide surface coated with single-domain antibody fragments. This format allows quick and efficient capture of distinct WBC subpopulations from small samples (∼30 μL) of whole blood in a geometry that does not require any specialized equipment such as cell sorters or microfluidic devices.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Whitehead Institute for Biomedical Research
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/chem.201503057
