Dynamic metabolic engineering: New strategies for developing responsive cell factories
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Prather_Dynamic metabolic.pdf
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430.81 KB
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Author(s)
Brockman, Irene Marie
Jones, Kristala L.
Date Issued
September 2015
Journal
Biotechnology Journal
Publisher
Wiley Blackwell
Citation
Brockman, Irene M. and Prather, Kristala L. J. “Dynamic Metabolic Engineering: New Strategies for Developing Responsive Cell Factories.” Biotechnology Journal 10, no. 9 (April 2015): 1360–1369 © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Version
Author's final manuscript
Abstract
Metabolic engineering strategies have enabled improvements in yield and titer for a variety of valuable small molecules produced naturally in microorganisms, as well as those produced via heterologous pathways. Typically, the approaches have been focused on up- and downregulation of genes to redistribute steady-state pathway fluxes, but more recently a number of groups have developed strategies for dynamic regulation, which allows rebalancing of fluxes according to changing conditions in the cell or the fermentation medium. This review highlights some of the recently published work related to dynamic metabolic engineering strategies and explores how advances in high-throughput screening and synthetic biology can support development of new dynamic systems. Dynamic gene expression profiles allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates. The implementation is more complex relative to static control, but advances in screening techniques and DNA synthesis will continue to drive innovation in this field.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1002/biot.201400422
