Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
Name
s41378-020-0169-8.pdf
Description
Published version
Size
827.67 KB
Format
Adobe PDF
Checksum (MD5)
1eab7ff416320584b4201f83351044b4
Author(s)
Nagarajan, Maxwell B
Tentori, Augusto M
Zhang, Wen Cai
Slack, Frank J
Doyle, Patrick S
Date Issued
2020
Journal
Microsystems and Nanoengineering
Publisher
Springer Science and Business Media LLC
Version
Final published version
Abstract
© 2020, The Author(s). Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-rasLSL-G12D/+; p53fl/fl). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/S41378-020-0169-8
