SMC complexes differentially compact mitotic chromosomes according to genomic context
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Mirny_Structural maintenance.pdf
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Author(s)
Schalbetter, Stephanie Andrea
Belton, Jon-Matthew
Miles, Catrina
Yu, Miao
Dekker, Job
Baxter, Jonathan
Goloborodko, Anton
Fudenberg, Geoffrey
Mirny, Leonid A
Alternative Title
SMC complexes differentially compact mitotic chromosomes according to genomic context
Date Issued
August 2017
Journal
Nature Cell Biology
Publisher
Nature Publishing Group
Citation
Schalbetter, Stephanie Andrea et al “SMC Complexes Differentially Compact Mitotic Chromosomes According to Genomic Context.” Nature Cell Biology 19, 9 (August 2017): 1071–1080 © 2017 Macmillan Publishers Limited, part of Springer Nature
Version
Author's final manuscript
Abstract
Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is to co-align sister chromatids, while condensin generally compacts mitotic chromosomes. We find strikingly different roles for the two complexes in budding yeast mitosis. First, cohesin is responsible for compacting mitotic chromosome arms, independently of sister chromatid cohesion. Polymer simulations demonstrate that this role can be fully accounted for through cis-looping of chromatin. Second, condensin is generally dispensable for compaction along chromosome arms. Instead, it plays a targeted role compacting the rDNA proximal regions and promoting resolution of peri-centromeric regions. Our results argue that the conserved mechanism of SMC complexes is to form chromatin loops and that distinct SMC-dependent looping activities are selectively deployed to appropriately compact chromosomes.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Physics
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/NCB3594
