Quantum dot/antibody conjugates for in vivo cytometric imaging in mice
Name
Han-2015-Quantum dot_antibody.pdf
Size
1.38 MB
Format
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Checksum (MD5)
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Author(s)
Han, Hee-Sun
Niemeyer, Elisabeth
Huang, Yuhui
Kamoun, Walid S.
Martin, John Daniel
Bhaumik, Jayeeta
Chen, Yunching
Roberge, Sylvie
Cui, Jian
Martin, Margaret R.
Date Issued
February 2015
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Han, Hee-Sun, Elisabeth Niemeyer, Yuhui Huang, Walid S. Kamoun, John D. Martin, Jayeeta Bhaumik, Yunching Chen, et al. “Quantum Dot/antibody Conjugates for in Vivo Cytometric Imaging in Mice.” Proc Natl Acad Sci USA 112, no. 5 (January 20, 2015): 1350–1355.
Version
Final published version
Abstract
Multiplexed, phenotypic, intravital cytometric imaging requires novel fluorophore conjugates that have an appropriate size for long circulation and diffusion and show virtually no nonspecific binding to cells/serum while binding to cells of interest with high specificity. In addition, these conjugates must be stable and maintain a high quantum yield in the in vivo environments. Here, we show that this can be achieved using compact (~15 nm in hydrodynamic diameter) and biocompatible quantum dot (QD) -Ab conjugates. We developed these conjugates by coupling whole mAbs to QDs coated with norbornene-displaying polyimidazole ligands using tetrazine–norbornene cycloaddition. Our QD immunoconstructs were used for in vivo single-cell labeling in bone marrow. The intravital imaging studies using a chronic calvarial bone window showed that our QD-Ab conjugates diffuse into the entire bone marrow and efficiently label single cells belonging to rare populations of hematopoietic stem and progenitor cells (Sca1[superscript +]c-Kit[superscript +] cells). This in vivo cytometric technique may be useful in a wide range of structural and functional imaging to study the interactions between cells and between a cell and its environment in intact and diseased tissues.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemistry
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1421632111
