Simultaneous Profiling of Gene Expression and Chromatin Accessibility in Single Cells
Name
nihms-1055401.pdf
Description
Accepted version
Size
654.17 KB
Format
Adobe PDF
Checksum (MD5)
fda9690330a3aeda8fc92e51569f894f
Author(s)
Reyes, Miguel
Blainey, Paul C
Date Issued
November 2019
Journal
Advanced biosystems
Publisher
Wiley
Citation
Reyes, Miguel et al. “Simultaneous Profiling of Gene Expression and Chromatin Accessibility in Single Cells.” Advanced biosystems 3 (2019) © 2019 The Author(s)
Version
Author's final manuscript
Abstract
Profiling multiple omic layers in a single cell enables the discovery and analysis of biological phenomena that are not apparent from analysis of mono-omic data. While methods for multiomic profiling have been reported, their adoption has been limited due to high cost and complex workflows. Here, a simple method for joint profiling of gene expression and chromatin accessibility in tens to hundreds of single cells is presented. Assessed herein is the quality of resulting single cell ATAC- and RNA-seq data across three cell types, examining the link between accessibility and expression at the CD3G and FTH1 loci in human primary T cells and monocytes, and comparing the accuracy of clustering solutions for mono-omic and combined data. The new method allows biological laboratories to perform simultaneous profiling of gene expression and chromatin accessibility using standard reagents and instrumentation. This technique, in conjunction with other advances in multiomic profiling, will enable highly resolved cell state classification and more specific mechanistic hypothesis generation than is possible with mono-omic analysis.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/ADBI.201900065
