Kinetochore asymmetry defines a single yeast lineage
Name
Thorpe-2009-Kinetochore asymmetr.pdf
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Author(s)
Thorpe, Peter H.
Bruno, Joanne H.
Rothstein, Rodney
Date Issued
April 2009
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences
Citation
Thorpe, Peter H, Joanne Bruno, and Rodney Rothstein. “Kinetochore asymmetry defines a single yeast lineage.” Proceedings of the National Academy of Sciences 106.16 (2009): 6673-6678.
Version
Final published version
Abstract
Asymmetric cell division is of fundamental importance in biology as it allows for the establishment of separate cell lineages during the development of multicellular organisms. Although microbial systems, including the yeast Saccharomyces cerevisiae, are excellent models of asymmetric cell division, this phenotype occurs in all cell divisions; consequently, models of lineage-specific segregation patterns in these systems do not exist. Here, we report the first example of lineage-specific asymmetric division in yeast. We used fluorescent tags to show that components of the yeast kinetochore, the protein complex that anchors chromosomes to the mitotic spindle, divide asymmetrically in a single postmeiotic lineage. This phenotype is not seen in vegetatively dividing haploid or diploid cells. This kinetochore asymmetry suggests a mechanism for the selective segregation of sister centromeres to daughter cells to establish different cell lineages or fates. These results provide a mechanistic link between lineage-defining asymmetry of metazoa with unicellular eukaryotes.
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DOI of Published Version
http://dx.doi.org/10.1073/pnas.0811248106
