High-resolution whole organ imaging using two-photon tissue cytometry
Name
Ragan-2007-High-resolution whol.pdf
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1.69 MB
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Checksum (MD5)
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Author(s)
Ragan, Timothy
Sylvan, Jeremy D.
Kim, Ki Hean
Huang, Hayden
Bahlmann, Karsten
Lee, Richard T.
So, Peter T. C.
Date Issued
February 2007
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Ragan, Timothy, Jeremy D. Sylvan, Ki Hean Kim, Hayden Huang, Karsten Bahlmann, Richard T. Lee, and Peter T. C. So. “High-Resolution Whole Organ Imaging Using Two-Photon Tissue Cytometry.” Journal of Biomedical Optics 12, no. 1 (2007): 014015. © 2007 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer’s disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1117/1.2435626
