DNA nanopores as artificial membrane channels for bioprotonics
Name
s41467-023-40870-1.pdf
Description
Published version
Size
1.67 MB
Format
Adobe PDF
Checksum (MD5)
553b729b65fc2d9e580db51d0b27bf1d
Author(s)
Luo, Le
Manda, Swathi
Park, Yunjeong
Demir, Busra
Sanchez, Jesse
Anantram, M. P.
Oren, Ersin Emre
Gopinath, Ashwin
Rolandi, Marco
Date Issued
September 4, 2023
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Citation
Luo, Le, Manda, Swathi, Park, Yunjeong, Demir, Busra, Sanchez, Jesse et al. 2023. "DNA nanopores as artificial membrane channels for bioprotonics." Nature Communications, 14 (1).
Version
Final published version
Abstract
AbstractBiological membrane channels mediate information exchange between cells and facilitate molecular recognition. While tuning the shape and function of membrane channels for precision molecular sensing via de-novo routes is complex, an even more significant challenge is interfacing membrane channels with electronic devices for signal readout, which results in low efficiency of information transfer - one of the major barriers to the continued development of high-performance bioelectronic devices. To this end, we integrate membrane spanning DNA nanopores with bioprotonic contacts to create programmable, modular, and efficient artificial ion-channel interfaces. Here we show that cholesterol modified DNA nanopores spontaneously and with remarkable affinity span the lipid bilayer formed over the planar bio-protonic electrode surface and mediate proton transport across the bilayer. Using the ability to easily modify DNA nanostructures, we illustrate that this bioprotonic device can be programmed for electronic recognition of biomolecular signals such as presence of Streptavidin and the cardiac biomarker B-type natriuretic peptide, without modifying the biomolecules. We anticipate this robust interface will allow facile electronic measurement and quantification of biomolecules in a multiplexed manner.
Subjects
General Physics and Astronomy
General Biochemistry, Genetics and Molecular Biology
General Chemistry
Multidisciplinary
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1038/s41467-023-40870-1
