Bistability of a coupled Aurora B kinase-phosphatase system in cell division
Name
Zaytsev-2016-Bistability of.pdf
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3.93 MB
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Author(s)
Godzi, Maxim
Calderon, Abram
Stamatov, Rumen
Zaytsev, Anatoly V.
Segura-Pena, Dario
Ballister, Edward R.
Mayo, Alyssa M.
Black, Ben E.
Ataullakhanov, Fazly I.
Lampson, Michael A.
Date Issued
January 2016
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
Zaytsev, Anatoly V, Dario Segura-Pena, Maxim Godzi, Abram Calderon, Edward R Ballister, Rumen Stamatov, Alyssa M Mayo, et al. “Bistability of a Coupled Aurora B Kinase-Phosphatase System in Cell Division.” eLife 5 (January 14, 2016).
Version
Final published version
Abstract
Aurora B kinase, a key regulator of cell division, localizes to specific cellular locations, but the regulatory mechanisms responsible for phosphorylation of substrates located remotely from kinase enrichment sites are unclear. Here, we provide evidence that this activity at a distance depends on both sites of high kinase concentration and the bistability of a coupled kinase-phosphatase system. We reconstitute this bistable behavior and hysteresis using purified components to reveal co-existence of distinct high and low Aurora B activity states, sustained by a two-component kinase autoactivation mechanism. Furthermore, we demonstrate these non-linear regimes in live cells using a FRET-based phosphorylation sensor, and provide a mechanistic theoretical model for spatial regulation of Aurora B phosphorylation. We propose that bistability of an Aurora B-phosphatase system underlies formation of spatial phosphorylation patterns, which are generated and spread from sites of kinase autoactivation, thereby regulating cell division.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.7554/eLife.10644
