Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy
Name
Raja-2010-Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy.pdf
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Author(s)
Raja, Anju M.
Xu, Shuoyu
Sun, Wanxin
Zhou, Jianbiao
Tai, Dean C. S.
Chen, Chien Shing
Rajapakse, Jagath
So, Peter T. C.
Yu, Hanry
Date Issued
October 2010
Journal
Journal of Biomedical Optics
Publisher
Society of Photo-optical Instrumentation Engineers
Citation
Raja, Anju M. et al. “Pulse-modulated Second Harmonic Imaging Microscope Quantitatively Demonstrates Marked Increase of Collagen in Tumor After Chemotherapy.” Journal of Biomedical Optics 15.5 (2010) : 056016. © 2010 SPIE.
Version
Final published version
Abstract
Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid leukemia (AML) tumor specimens treated with a new drug against receptor tyrosine kinase (ABT-869), and observe a significant increase in collagen area percentage, collagen fiber length, fiber width, and fiber number after chemotherapy. This finding reveals new insights into tumor responses to chemotherapy and suggests caution in developing new drugs and therapeutic regimens against cancers.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1117/1.3497565
