Gastrointestinal synthetic epithelial linings
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abc0441_Li et al_ACCEPTED.pdf
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6.64 MB
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Author(s)
LI, Junwei
Wang, Thomas
Shi, Yunhua
Jones, Alexis
Moussa, Zaina
Lopes, Aaron
Collins, Joy
Tamang, Siddartha M
Hess, Kaitlyn
Shakur, Rameen
Date Issued
2020
Journal
Science Translational Medicine
Publisher
American Association for the Advancement of Science
Citation
Li, Junwei et al. “Gastrointestinal synthetic epithelial linings.” Science Translational Medicine, 12, (2020) © 2020 The Author(s)
Version
Author's final manuscript
Abstract
Epithelial tissuesline the organs of the body, providing an initial protective barrieras well as a surface for nutrient and drug absorption. Here we identifiedenzymatic components present in the gastrointestinal epithelium that canserve as selective means for tissue-directed polymerization. We focusedon the small intestine, given its role in drug and nutrient absorption, and identified catalase as an essential enzyme with the potential to catalyze polymerization and growth of synthetic biomaterial layers. We demonstrated that the olymerization of dopamine by catalase yieldsstrong tissueadhesion. We characterizedthe mechanism and specificity of the polymerization in segments of the gastrointestinal tracts of pigs and human sex vivo. Moreover, we demonstrated proof-of-concept for application of these gastrointestinal synthetic epithelial linings(GSELs)for drug delivery, enzymatic immobilizationfor digestive supplementation, and nutritional modulation through transient barrier formationin pigs.This catalase-based approach to insitu biomaterial generationmay have broad indicationsfor gastrointestinal applications.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Division of Comparative Medicine
Massachusetts Institute of Technology. Department of Mechanical Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://stm.sciencemag.org/content/12/558/eabc0441
