Organization of the Mitotic Chromosome
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Author(s)
Naumova, Natalia
Zhan, Ye
Lajoie, Bryan R.
Dekker, Job
Imakaev, Maksim Viktorovich
Fudenberg, Geoffrey
Mirny, Leonid A
Date Issued
November 2013
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Naumova, Natalia et al. "Organization of the Mitotic Chromosome." Science 342, 6161 (November 2013): 948-953 © 2013 American Association for the Advancement of Science
Version
Author's final manuscript
Abstract
Mitotic chromosomes are among the most recognizable structures in the cell, yet for over a century their internal organization remains largely unsolved. We applied chromosome conformation capture methods, 5C and Hi-C, across the cell cycle and revealed two alternative three-dimensional folding states of the human genome. We show that the highly compartmentalized and cell-type-specific organization described previously for non-synchronous cells is restricted to interphase. In metaphase, we identify a homogenous folding state, which is locus-independent, common to all chromosomes, and consistent among cell types, suggesting a general principle of metaphase chromosome organization. Using polymer simulations, we find that metaphase Hi-C data are inconsistent with classic hierarchical models, and is instead best described by a linearly-organized longitudinally compressed array of consecutive chromatin loops.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Physics
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
http://science.sciencemag.org/content/342/6161/948
