On-chip multi-dimensional biomolecule separation using multi-layer microfabricated valves
Name
56889960-MIT.pdf
Description
Full printable version
Size
5.42 MB
Format
Adobe PDF
Checksum (MD5)
63a629131e8afc716f1310fa7e79b2ba
Author(s)
Wang, Ying-Chih, 1977-
Advisor(s)
Jongyoon Han.
Date Issued
2004
Publisher
Massachusetts Institute of Technology
Abstract
Recent proteomics researches require a sensitive, high-speed, and automatic protein separation tool that far exceeds the capability of traditional two dimensional (2D) protein gel electrophoresis. Methods are described to achieve multi-dimensional biomolecule separation in a microfluidic channel. The new methods couple isoelectric focusing (IEF) with high ionic strength capillary electrophoresis (CE) by active micro valve control in a microchip. Several experiments demonstrating independent 2D separation were performed, and critical parameters for better chip performance were identified; including channel passivation, electroosmosis control, IEF linearity control, and detection enhancement. The result can be used for the filtration of high-abundance proteins, which used to be done by affinity columns. Also, it can be used for much possible integration between different heterogeneous separation/analysis techniques such as IEF, polyacrylamide gel electrophoresis (PAGE), CE, reverse-phase chromatography, and mass spectrometry (MS).
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.
Includes bibliographical references (p. 89-94).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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