Upregulation of μ3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway
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Steinmetz-2016-Upregulation of mu3A.pdf
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2.52 MB
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Author(s)
Steinmetz, Celine C.
Tatavarty, Vedakumar
Sugino, Ken
Shima, Yasuyuki
Joseph, Anne
Lin, Heather
Rutlin, Michael
Hempel, Chris M.
Okaty, Benjamin W.
Paradis, Suzanne
Date Issued
August 2016
Journal
Cell Reports
Publisher
Elsevier
Citation
Steinmetz, Celine C. et al. “Upregulation of μ3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.” Cell Reports 16.10 (2016): 2711–2722.
Version
Final published version
Abstract
Synaptic scaling is a form of homeostatic plasticity driven by transcription-dependent changes in AMPA-type glutamate receptor (AMPAR) trafficking. To uncover the pathways involved, we performed a cell-type-specific screen for transcripts persistently altered during scaling, which identified the μ subunit (μ3A) of the adaptor protein complex AP-3A. Synaptic scaling increased μ3A (but not other AP-3 subunits) in pyramidal neurons and redistributed dendritic μ3A and AMPAR to recycling endosomes (REs). Knockdown of μ3A prevented synaptic scaling and this redistribution, while overexpression (OE) of full-length μ3A or a truncated μ3A that cannot interact with the AP-3A complex was sufficient to drive AMPAR to REs. Finally, OE of μ3A acted synergistically with GRIP1 to recruit AMPAR to the dendritic membrane. These data suggest that excess μ3A acts independently of the AP-3A complex to reroute AMPAR to RE, generating a reservoir of receptors essential for the regulated recruitment to the synaptic membrane during scaling up.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1016/j.celrep.2016.08.009
