Directed evolution in mammalian cells
Name
nihms-1686869.pdf
Description
Accepted version
Size
1.95 MB
Format
Unknown
Checksum (MD5)
e0c3581c9c530f13a2f077d30d7853f7
Author(s)
Hendel, Samuel J
Shoulders, Matthew D.
Date Issued
2021
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Hendel, Samuel J and Shoulders, Matthew D. 2021. "Directed evolution in mammalian cells." Nature Methods, 18 (4).
Version
Author's final manuscript
Abstract
Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast-even when the goal is to probe or modulate mammalian biology. Performing directed evolution in systems that do not match the intended mammalian environment severely constrains the scope and functionality of the targets that can be evolved. We review new platforms that are now making it possible to use the mammalian cell itself as the setting for directed evolution and present an overview of frontier challenges and high-impact targets for this approach.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/S41592-021-01090-X
