Design and fabrication of a microfluidies gradient generator system for high-throughput molecular interaction studies
Name
57351170-MIT.pdf
Size
30.94 MB
Format
Adobe PDF
Checksum (MD5)
b5e5503f949099f51d4dbc1c42a22f20
Author(s)
Chen, Guan-Jong,
1981-
Date Issued
2004
Publisher
Massachusetts Institute of Technology
Abstract
Design and fabrication of a microfluidics system capable of generating reproducible and controlled micro-biochemical environments that can be used as a diagnostic assay and microreactor is important. Here, a simple technique was developed to create a robust microfluidics system capable of generating precise gradients of biochemical properties within its channels. Through this approach, it is possible to create a gradient generator with mammalian cells patterned and seeded under its poly(dimethylsiloxane) (PDMS) channels. Cells that were seeded and patterned under the PDMS channels remained viable and capable of performing intracellular reactions. Using the gradient generator within the PDMS microfluidic device, a gradient of specific and controlled biochemicals can be flowed on seeded cells allowing for high-throughput molecular interaction analysis. The microfluidics system provides a way to study and analyze cell response in the presence of a combination of biochemical signals.
Description
Thesis: S.M., Massachusetts Institute of Technology, Biological Engineering Division, 2004
Includes bibliographical references (leaves 45-47).
Subjects
Biological Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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