In Vivo Imaging with a Cell-Permeable Porphyrin-Based MRI Contrast
Name
Jasanoff_In vivo imaging_NIH Paper_DF.pdf
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1.24 MB
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Author(s)
Lee, Taekwan
Zhang, Xiao-an
Dhar, Shanta
Faas, Henryk
Lippard, Stephen J.
Jasanoff, Alan Pradip
Date Issued
June 2010
Journal
Chemistry and Biology
Publisher
Elsevier B.V.
Citation
Lee, Taekwan et al. “In Vivo Imaging with a Cell-Permeable Porphyrin-Based MRI Contrast Agent.” Chemistry & Biology 17 (2010): 665-673.
Version
Author's final manuscript
Abstract
Magnetic resonance imaging (MRI) with molecular probes offers the potential to monitor physiological parameters with comparatively high spatial and temporal resolution in living subjects. For detection of intracellular analytes, construction of cell-permeable imaging agents remains a challenge. Here we show that a porphyrin-based MRI molecular imaging agent, Mn-(DPA-C2)2-TPPS3, effectively penetrates cells and persistently stains living brain tissue in intracranially injected rats. Chromogenicity of the probe permitted direct visualization of its distribution by histology, in addition to MRI. Distribution was concentrated in cell bodies after hippocampal infusion. Mn-(DPA-C2)2-TPPS3 was designed to sense zinc ions, and contrast enhancement was more pronounced in the hippocampus, a zinc-rich brain region, than in the caudate nucleus, which contains relatively little labile Zn2+. Membrane permeability, optical activity, and high relaxivity of porphyrin-based contrast agents offer exceptional functionality for in vivo imaging.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Nuclear Science and Engineering
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1016/j.chembiol.2010.05.009
