Polypeptide translocation by the AAA+ ClpXP protease machine
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Baker_Polypeptide translocation.pdf
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Author(s)
Barkow, Sarah R.
Levchenko, Igor
Baker, Tania
Sauer, Robert T
Date Issued
June 2009
Journal
Chemistry and Biology
Publisher
Elsevier
Citation
Barkow, Sarah R. et al. “Polypeptide Translocation by the AAA+ ClpXP Protease Machine.” Chemistry & Biology 16.6 (2009): 605–612.
Version
Author's final manuscript
Abstract
In the AAA+ ClpXP protease, ClpX uses repeated cycles of ATP hydrolysis to pull native proteins apart and to translocate the denatured polypeptide into ClpP for degradation. Here, we probe polypeptide features important for translocation. ClpXP degrades diverse synthetic peptide substrates despite major differences in side-chain chirality, size, and polarity. Moreover, translocation occurs without a peptide –NH and with 10 methylenes between successive peptide bonds. Pulling on homopolymeric tracts of glycine, proline, and lysine also allows efficient ClpXP degradation of a stably folded protein. Thus, minimal chemical features of a polypeptide chain are sufficient for translocation and protein unfolding by the ClpX machine. These results suggest that the translocation pore of ClpX is highly elastic, allowing interactions with a wide range of chemical groups, a feature likely to be shared by many AAA+ unfoldases.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1016/j.chembiol.2009.05.007
