Isolation and retrieval of circulating tumor cells using centrifugal forces
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Han_Isolation and.pdf
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1.29 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Hou, Han Wei
Warkiani, Majid Ebrahimi
Khoo, Bee Luan
Li, Zi Rui
Soo, Ross A.
Tan, Daniel Shao-Weng
Lim, Wan-Teck
Han, Jongyoon
Bhagat, Ali Asgar S.
Lim, Chwee Teck
Date Issued
February 2013
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Hou, Han Wei, Majid Ebrahimi Warkiani, Bee Luan Khoo, Zi Rui Li, Ross A. Soo, Daniel Shao-Weng Tan, Wan-Teck Lim, Jongyoon Han, Ali Asgar S. Bhagat, and Chwee Teck Lim. “Isolation and Retrieval of Circulating Tumor Cells Using Centrifugal Forces.” Sci. Rep. 3 (February 12, 2013).
Version
Final published version
Abstract
Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5–88 CTCs per mL). The presence of CD133+ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License
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DOI of Published Version
https://doi.org/10.1038/srep01259
