Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi)
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fevo-09-676682.pdf
Description
Published version
Size
3.78 MB
Format
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Checksum (MD5)
80934937fc42897d3d014a72713305c0
Author(s)
Chan, Nathan
Shen, Zeli
Mannion, Anthony
Kurnick, Susanna
Popescu, Ioana S
Burton, Frederic J
Calle, Paul P
Fox, James G
Date Issued
September 14, 2021
Journal
Frontiers in Ecology and Evolution
Publisher
Frontiers Media SA
Citation
Chan, Nathan, Shen, Zeli, Mannion, Anthony, Kurnick, Susanna, Popescu, Ioana S et al. 2021. "Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi)." Frontiers in Ecology and Evolution, 9.
Version
Final published version
Abstract
Blue iguanas (Cyclura lewisi) are endangered reptiles found only on Grand Cayman. Previously, DNA for a novel Helicobacter species GCBI1 was detected in sick and dead iguanas. In the current study, fecal and cloacal swab samples were obtained from 25 iguanas. Through molecular and microbiological techniques, a novel Helicobacter species was cultured from feces and characterized, for whom we propose the name Helicobacter cyclurae. This novel helicobacter had a prevalence of 56% by PCR and 20% by culture in samples analyzed. The type strain MIT 16-1353 was catalase, oxidase, and gamma-glutamyl transpeptidase positive. By electron microscopy, H. cyclurae has a curved rod morphology and a single sheathed polar flagellum. Phylogenetic analysis using 16S rRNA, gyrB, and hsp60 indicated that these strains were most closely related to Helicobacter sp. 12502256-12 previously isolated from lizards. H. cyclurae has a 1.91-Mb genome with a GC content of 33.37%. There were 1,969 genes with four notable virulence genes: high temperature requirement-A protein-secreted serine protease, gamma-glutamyl transpeptidase, fibronectin/fibrinogen binding protein, and neutrophil-activating protein. Whole-genome phylogeny, average nucleotide identity, and digital DNA–DNA hybridization analysis confirmed that H. cyclurae is a novel species, and the first helicobacter cultured and characterized from blue iguanas.
MIT Department
Massachusetts Institute of Technology. Division of Comparative Medicine
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Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.3389/fevo.2021.676682
