Development of a cellulose-based 96-well plate vertical flow pull-down assay
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D3AY00614J.pdf
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Author(s)
Cheng, Hoi Lok
Jia, Huan
Lim, Sing Mei
Ng, Say Yong
Kongsuphol, Patthara
McBee, Megan E.
Sikes, Hadley D.
Date Issued
2023
Journal
Analytical Methods
Publisher
Royal Society of Chemistry
Citation
Cheng, Hoi Lok, Jia, Huan, Lim, Sing Mei, Ng, Say Yong, Kongsuphol, Patthara et al. 2023. "Development of a cellulose-based 96-well plate vertical flow pull-down assay." Analytical Methods, 15 (28).
Version
Final published version
Abstract
The abundance and low production cost of biomaterial cellulose paper have attracted attention for many applications. Point-of-care (PoC) diagnostic tests have been successfully developed using patterned cellulose paper. Although PoC diagnostic tests are rapid and simple to perform, their sample processing throughput is limited, allowing for only one sample to be evaluated at a time, which restricts potential applications. Thus, it was appealing to expand cellulose-based PoC tests to high-throughput versions to increase their applicability. Here, we present the development of a high-throughput cellulose-based 96-well plate vertical flow pull-down assay that can process 96 tests, is easy to prepare, and can be customized for different detection targets. The device has two key features: (i) patterned cellulose paper for 96 tests that do not require pre-immobilization of capturing reagents, and (ii) reusable sturdy housing. We believe that a variety of applications, including laboratory testing, population surveillance tests, and sizable clinical trials for diagnostic tests, can benefit from the adoption of this cellulose-based 96-well plate assay.
Subjects
General Engineering
General Chemical Engineering
Analytical Chemistry
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1039/d3ay00614j
