Monosynaptic tracing: a step-by-step protocol
Name
Monosynaptic tracing protocol__16 with figs and drawings (small).pdf
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342.23 KB
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Adobe PDF
Checksum (MD5)
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Author(s)
Lavin, Thomas K.
Jin, Lei
Wickersham, Ian R.
Date Issued
December 2019
Journal
Journal of Chemical Neuroanatomy
Publisher
Elsevier BV
Citation
Lavin, Thomas K. et al. "Monosynaptic tracing: a step-by-step protocol." Journal of Chemical Neuroanatomy 102 (December 2019): 101661. © 2019 Elsevier B.V.
Version
Author's final manuscript
Abstract
Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors.
MIT Department
McGovern Institute for Brain Research at MIT
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.jchemneu.2019.101661
