A variably imprinted epiallele impacts seed development
Name
journal.pgen.1007469.pdf
Description
Published version
Size
3.42 MB
Format
Adobe PDF
Checksum (MD5)
15d0c7740bd0b5d74c7d22a291231206
Author(s)
Pignatta, Daniela
Novitzky, Katherine
Satyaki, P. R. V.
Gehring, Mary
Date Issued
November 2018
Journal
PLoS Genetics
Publisher
Public Library of Science (PLoS)
Citation
Pignatta, Daniela et al. "A variably imprinted epiallele impacts seed development." PLoS Genetics 14, 11 (November 2018): e1007469 © 2018 Pignatta et al
Version
Final published version
Abstract
The contribution of epigenetic variation to phenotypic variation is unclear. Imprinted genes, because of their strong association with epigenetic modifications, represent an opportunity for the discovery of such phenomena. In mammals and flowering plants, a subset of genes are expressed from only one parental allele in a process called gene imprinting. Imprinting is associated with differential DNA methylation and chromatin modifications between parental alleles. In flowering plants imprinting occurs in a seed tissue - endosperm. Proper endosperm development is essential for the production of viable seeds. We previously showed that in Arabidopsis thaliana intraspecific imprinting variation is correlated with naturally occurring DNA methylation polymorphisms. Here, we investigated the mechanisms and function of allele-specific imprinting of the class IV homeodomain leucine zipper (HD-ZIP) transcription factor HDG3. In imprinted strains, HDG3 is expressed primarily from the methylated paternally inherited allele. We manipulated the methylation state of endogenous HDG3 in a non-imprinted strain and demonstrated that methylation of a proximal transposable element is sufficient to promote HDG3 expression and imprinting. Gain of HDG3 imprinting was associated with earlier endosperm cellularization and changes in seed weight. These results indicate that epigenetic variation alone is sufficient to explain imprinting variation and demonstrate that epialleles can underlie variation in seed development phenotypes.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1371/JOURNAL.PGEN.1007469
