The Efficacy of an Immunoisolating Membrane System for Islet Xenotransplantation in Minipigs
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Neufeld-2013-The Efficacy of an I.pdf
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Author(s)
Neufeld, Tova
Ludwig, Barbara
Barkai, Uriel
Weir, Gordon C.
Colton, Clark K.
Evron, Yoav
Balyura, Maria
Yavriyants, Karina
Zimermann, Baruch
Azarov, Dmitri
Date Issued
August 2013
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Neufeld, Tova, Barbara Ludwig, Uriel Barkai, Gordon C. Weir, Clark K. Colton, Yoav Evron, Maria Balyura, et al. “The Efficacy of an Immunoisolating Membrane System for Islet Xenotransplantation in Minipigs.” Edited by Kathrin Maedler. PLoS ONE 8, no. 8 (August 1, 2013): e70150.
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Final published version
Abstract
Developing a device that protects xenogeneic islets to allow treatment and potentially cure of diabetes in large mammals has been a major challenge in the past decade. Using xenogeneic islets for transplantation is required in light of donor shortage and the large number of diabetic patients that qualify for islet transplantation. Until now, however, host immunoreactivity against the xenogeneic graft has been a major drawback for the use of porcine islets. Our study demonstrates the applicability of a novel immunoprotective membrane that allows successful xenotransplantation of rat islets in diabetic minipigs without immunosuppressive therapy. Rat pancreatic islets were encapsulated in highly purified alginate and integrated into a plastic macrochamber covered by a poly-membrane for subcutaneous transplantation. Diabetic Sinclair pigs were transplanted and followed for up to 90 days. We demonstrated a persistent graft function and restoration of normoglycemia without the need for immunosuppressive therapy. This concept could potentially offer an attractive strategy for a more widespread islet replacement therapy that would restore endogenous insulin secretion in diabetic patients without the need for immunosuppressive drugs and may even open up an avenue for safe utilization of xenogeneic islet donors.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0070150
