RBP Image Database: A resource for the systematic characterization of the subcellular distribution properties of human RNA binding proteins
Name
gkac971.pdf
Description
Published version
Size
4.76 MB
Format
Adobe PDF
Checksum (MD5)
4f21878c161d977e5ab88fba51c9c1bc
Author(s)
Benoit Bouvrette, Louis Philip
Wang, Xiaofeng
Boulais, Jonathan
Kong, Jian
Syed, Easin Uddin
Blue, Steven M
Zhan, Lijun
Olson, Sara
Stanton, Rebecca
Wei, Xintao
Date Issued
November 2, 2022
Journal
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Citation
Benoit Bouvrette, Louis Philip, Wang, Xiaofeng, Boulais, Jonathan, Kong, Jian, Syed, Easin Uddin et al. 2022. "RBP Image Database: A resource for the systematic characterization of the subcellular distribution properties of human RNA binding proteins." Nucleic Acids Research.
Version
Final published version
Abstract
Abstract
RNA binding proteins (RBPs) are central regulators of gene expression implicated in all facets of RNA metabolism. As such, they play key roles in cellular physiology and disease etiology. Since different steps of post-transcriptional gene expression tend to occur in specific regions of the cell, including nuclear or cytoplasmic locations, defining the subcellular distribution properties of RBPs is an important step in assessing their potential functions. Here, we present the RBP Image Database, a resource that details the subcellular localization features of 301 RBPs in the human HepG2 and HeLa cell lines, based on the results of systematic immuno-fluorescence studies conducted using a highly validated collection of RBP antibodies and a panel of 12 markers for specific organelles and subcellular structures. The unique features of the RBP Image Database include: (i) hosting of comprehensive representative images for each RBP-marker pair, with ∼250,000 microscopy images; (ii) a manually curated controlled vocabulary of annotation terms detailing the localization features of each factor; and (iii) a user-friendly interface allowing the rapid querying of the data by target or annotation. The RBP Image Database is freely available at https://rnabiology.ircm.qc.ca/RBPImage/.
RNA binding proteins (RBPs) are central regulators of gene expression implicated in all facets of RNA metabolism. As such, they play key roles in cellular physiology and disease etiology. Since different steps of post-transcriptional gene expression tend to occur in specific regions of the cell, including nuclear or cytoplasmic locations, defining the subcellular distribution properties of RBPs is an important step in assessing their potential functions. Here, we present the RBP Image Database, a resource that details the subcellular localization features of 301 RBPs in the human HepG2 and HeLa cell lines, based on the results of systematic immuno-fluorescence studies conducted using a highly validated collection of RBP antibodies and a panel of 12 markers for specific organelles and subcellular structures. The unique features of the RBP Image Database include: (i) hosting of comprehensive representative images for each RBP-marker pair, with ∼250,000 microscopy images; (ii) a manually curated controlled vocabulary of annotation terms detailing the localization features of each factor; and (iii) a user-friendly interface allowing the rapid querying of the data by target or annotation. The RBP Image Database is freely available at https://rnabiology.ircm.qc.ca/RBPImage/.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
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DOI of Published Version
https://doi.org/10.1093/nar/gkac971
