DNA sense-and-respond protein modules for mammalian cells
Name
DNA sense-and-respond protein modules for mammalian cells-Collins.pdf
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4.95 MB
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Author(s)
Slomovic, Shimyn
Collins, James J.
Date Issued
September 2015
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Slomovic, Shimyn and Collins, James J. “DNA Sense-and-Respond Protein Modules for Mammalian Cells.” Nature Methods 12, no. 11 (September 21, 2015): 1085–1090. © 2015 Macmillan Publishers Limited, part of Springer Nature
Version
Author's final manuscript
Abstract
We generated synthetic protein components that can detect specific DNA sequences and subsequently trigger a desired intracellular response. These modular sensors exploit the programmability of zinc-finger DNA recognition to drive the intein-mediated splicing of an artificial trans-activator that signals to a genetic circuit containing a given reporter or response gene. We used the sensors to mediate sequence recognition−induced apoptosis as well as to detect and report a viral infection. This work establishes a synthetic biology framework for endowing mammalian cells with sentinel capabilities, which provides a programmable means to cull infected cells. It may also be used to identify positively transduced or transfected cells, isolate recipients of intentional genomic edits and increase the repertoire of inducible parts in synthetic biology.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmeth.3585
