Evaluating anesthetic protocols for functional blood flow imaging in the rat eye
Name
Moult-2017-Evaluating anesthetic protocols for.pdf
Size
721.57 KB
Format
Adobe PDF
Checksum (MD5)
f467b674640cde6652a889ccb797c8b6
Author(s)
Choi, WooJhon
Boas, David A.
Clermont, Allen C.
Feener, Edward P.
Moult, Eric Michael
Baumann, Bernhard
Fujimoto, James G
Date Issued
January 2017
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Moult, Eric M.; Choi, WooJhon; Boas, David A.; Baumann, Bernhard; Clermont, Allen C.; Feener, Edward P. and Fujimoto, James G. “Evaluating Anesthetic Protocols for Functional Blood Flow Imaging in the Rat Eye.” Journal of Biomedical Optics 22, no. 1 (January 2017): 016005 © 2016 The Authors
Version
Final published version
Abstract
The purpose of this study is to evaluate the suitability of five different anesthetic protocols (isoflurane, isoflurane–xylazine, pentobarbital, ketamine–xylazine, and ketamine–xylazine–vecuronium) for functional blood flow imaging in the rat eye. Total retinal blood flow was measured at a series of time points using an ultrahigh-speed Doppler OCT system. Additionally, each anesthetic protocol was qualitatively evaluated according to the following criteria: (1) time-stability of blood flow, (2) overall rate of blood flow, (3) ocular immobilization, and (4) simplicity. We observed that different anesthetic protocols produced markedly different blood flows. Different anesthetic protocols also varied with respect to the four evaluated criteria. These findings suggest that the choice of anesthetic protocol should be carefully considered when designing and interpreting functional blood flow studies in the rat eye.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1117/1.jbo.22.1.016005
