Cellular anatomy of the mouse primary motor cortex
Name
s41586-021-03970-w.pdf
Description
Published version
Size
41.18 MB
Format
Adobe PDF
Checksum (MD5)
8c95ebe52b7d275cd4a4b94e48ba2b7e
Date Issued
October 6, 2021
Journal
Nature
Publisher
Springer Science and Business Media LLC
Citation
Muñoz-Castañeda, R., Zingg, B., Matho, K.S. et al. Cellular anatomy of the mouse primary motor cortex. Nature 598, 159–166 (2021).
Version
Final published version
Abstract
An essential step toward understanding brain function is to establish a structural framework with cellular resolution on which multi-scale datasets spanning molecules, cells, circuits and systems can be integrated and interpreted1. Here, as part of the collaborative Brain Initiative Cell Census Network (BICCN), we derive a comprehensive cell type-based anatomical description of one exemplar brain structure, the mouse primary motor cortex, upper limb area (MOp-ul). Using genetic and viral labelling, barcoded anatomy resolved by sequencing, single-neuron reconstruction, whole-brain imaging and cloud-based neuroinformatics tools, we delineated the MOp-ul in 3D and refined its sublaminar organization. We defined around two dozen projection neuron types in the MOp-ul and derived an input–output wiring diagram, which will facilitate future analyses of motor control circuitry across molecular, cellular and system levels. This work provides a roadmap towards a comprehensive cellular-resolution description of mammalian brain architecture.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Chemical Engineering
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Creative Commons Attribution
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DOI of Published Version
10.1038/s41586-021-03970-w
