An Exported Peptide Functions Intracellularly to Contribute to Cell Density Signaling in B. subtilis
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Grossman_An exported.pdf
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Author(s)
Lazazzera, Beth A.
Solomon, Jonathan M.
Grossman, Alan Davis
Date Issued
June 1997
Journal
Cell
Publisher
Elsevier
Citation
Lazazzera, B. “An Exported Peptide Functions Intracellularly to Contribute to Cell Density Signaling in B. Subtilis.” Cell 89.6 (1997): 917–925. Copyright © 1997 Cell Press
Version
Final published version
Abstract
Competence development and sporulation in B. subtilis are partly controlled by peptides that accumulate in culture medium as cells grow to high density. We constructed two genes that encode mature forms of two different signaling molecules, the PhrA peptide that stimulates sporulation, and CSF, the competence- and sporulation-stimulating factor. Both pentapeptides are normally produced by secretion and processing of precursor molecules. The mature pentapeptides were functional when expressed inside the cell, indicating that they normally need to be imported to function. Furthermore, at physiological concentrations (10 nM), CSF was transported into the cell by the oligopeptide permease encoded by spo0K (opp). CSF was shown to have at least three different targets corresponding to its three activities: stimulating competence gene expression at low concentrations, and inhibiting competence gene expression and stimulating sporulation at high concentrations.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1016/S0092-8674(00)80277-9
