Interaction of amphiphilic nanoparticles with structurally perturbed lipid membranes
Name
1121596567-MIT.pdf
Size
3.89 MB
Format
Adobe PDF
Checksum (MD5)
7782ce1be7c726b54f6f6715b8c39446
Author(s)
Derry, Alexander(Alexander W.)
Advisor(s)
Alfredo Alexander-Katz.
Date Issued
2018
Publisher
Massachusetts Institute of Technology
Abstract
Understanding the interactions between nanoparticles and lipid membranes is important for applications such as drug delivery and membrane-protein mimetics. Perturbations such as area asymmetry and lateral tension affect these interactions by inducing various structural changes to the membranes. We use molecular dynamics simulations to demonstrate that the introduction of area asymmetry to a bilayer membrane significantly decreases the insertion latency of amphiphilic gold nanoparticles into both the densely and sparsely packed leaflets. We further demonstrate using transition state analysis that the dominant mechanisms for insertion into the dense and sparse leaflets are lipid desorption and lipid tail protrusions, respectively. These findings are supported by potential of mean force calculations showing that the energy barrier to protrusion is lower in the sparse leaflet, while that of desorption is lower in the dense leaflet. We also demonstrate that the structural characteristics of the bilayer when subject to lateral tension are similar to that observed in membranes with area asymmetry, suggesting a similar reduction in insertion latency. Further, we observe that lateral tension also increases the likelihood of nanoparticle ligands flipping across the bilayer, which is necessary for the nanoparticle adopting a stable symmetric configuration in the membrane.
Description
This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Thesis: S.B., Massachusetts Institute of Technology, Department of Materials Science and Engineering, 2018
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 43-45).
Subjects
Materials Science and Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Terms of Use
MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.
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