Protection of Anaerobic Microbes from Processing Stressors Using Metal–Phenolic Networks
Name
fan-et-al-2021-protection-of-anaerobic-microbes-from-processing-stressors-using-metal-phenolic-networks.pdf
Description
Published version
Size
4.48 MB
Format
Adobe PDF
Checksum (MD5)
3a5a490a8c796bdcf17936d78e7a5dca
Author(s)
Fan, Gang
Wasuwanich, Pris
Rodriguez-Otero, Mariela R
Furst, Ariel L
Date Issued
February 16, 2022
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Gang Fan, Pris Wasuwanich, Mariela R. Rodriguez-Otero, and Ariel L. Furst
Journal of the American Chemical Society 2022 144 (6), 2438-2443.
Version
Final published version
Abstract
The gut microbiome is essential to maintain overall health and prevent disease, which can occur when these microbes are not in homeostasis. Microbial biotherapeutics are important to combat these issues, but they must be alive at the time of delivery for efficacy. Many potentially therapeutic species are anaerobes and thus are difficult to manufacture because of the limited efficacy of existing protective methods, making their production nearly impossible. We have developed a self-assembling cellular coating to improve the viability and stability of the next-generation biotherapeutic Bacteroides thetaiotaomicron. We show protection from both harsh processing conditions and oxygen exposure, even in the absence of canonical cryoprotectants. This advance will increase the range of microbes that can be stably manufactured and facilitate the development of emerging strains of interest by ensuring their postproduction viability.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Center for Environmental Health Sciences
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivatives
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1021/jacs.1c09018
