Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator
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Han_Enhancing protease.pdf
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973.5 KB
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Checksum (MD5)
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Author(s)
Chen, Chia-Hung
Sarkar, Aniruddh
Song, Yong-Ak
Miller, Miles Aaron
Kim, Sung Jae
Griffith, Linda G.
Lauffenburger, Douglas A.
Han, Jongyoon
Date Issued
June 2011
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society (ACS)
Citation
Chen, Chia-Hung et al. “Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator.” Journal of the American Chemical Society 133.27 (2011): 10368–10371.
Version
Author's final manuscript
Abstract
We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1021/ja2036628
