New approaches to understanding the spatial organization of bacterial genomes
Name
New approaches.pdf
Size
907.75 KB
Format
Adobe PDF
Checksum (MD5)
23cc0e651710eff7030833774870d93e
Author(s)
Le, Tung
Laub, Michael T
Date Issued
October 2014
Journal
Current Opinion in Microbiology
Publisher
Elsevier
Citation
Le, Tung BK, and Michael T Laub. “New Approaches to Understanding the Spatial Organization of Bacterial Genomes.” Current Opinion in Microbiology 22 (December 2014): 15–21.
Version
Author's final manuscript
Abstract
In all organisms, chromosomal DNA must be compacted nearly three orders of magnitude to fit within the limited volume of a cell. However, chromosomes cannot be haphazardly packed, and instead must adopt structures compatible with numerous cellular processes, including DNA replication, chromosome segregation, recombination, and gene expression. Recent technical advances have dramatically enhanced our understanding of how chromosomes are organized in vivo and have begun to reveal the mechanisms and forces responsible. Here, we review the current arsenal of techniques used to query chromosome structure, focusing first on single-cell fluorescence microscopy approaches that directly examine chromosome structure and then on population-averaged biochemical methods that infer chromosome structure based on the interaction frequencies of different loci. We describe the power of these techniques, highlighting the major advances they have produced while also discussing their limitations.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.mib.2014.09.014
