Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions
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Saha-2011-Surface-engineered s.pdf
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Author(s)
Saha, Krishanu
Mei, Ying
Reisterer, Colin M.
Pyzocha, Neena Kenton
Yang, Jing
Muffat, Julien
Davies, Martyn C.
Alexander, Morgan R.
Jaenisch, Rudolf
Langer, Robert S
Date Issued
November 2011
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Citation
Saha, K. et al. “Surface-engineered Substrates for Improved Human Pluripotent Stem Cell Culture Under Fully Defined Conditions.” Proceedings of the National Academy of Sciences 108.46 (2011): 18714–18719. Copyright ©2011 by the National Academy of Sciences
Version
Final published version
Abstract
The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human pluripotent stem cell culture. Chemical and geometrical optimization of the surfaces enables control of early cell aggregation from fully dissociated cells, as predicted from a numerical model of cell migration, and results in significant increases in cell growth of undifferentiated cells. These chemically defined xeno-free substrates generate more than three times the number of cells than feeder-containing substrates per surface area. Further, reprogramming and typical gene-targeting protocols can be readily performed on these engineered surfaces. These substrates provide an attractive cell culture platform for the production of clinically relevant factor-free reprogrammed cells from patient tissue samples and facilitate the definition of standardized scale-up friendly methods for disease modeling and cell therapeutic applications.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1114854108
