Rapid activation of distinct members of multigene families in Plasmodium spp
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s42003-020-1081-3.pdf
Description
Published version
Size
2 MB
Format
Adobe PDF
Checksum (MD5)
80d382c90b2a2f8e83542547ecc518fe
Author(s)
Omelianczyk, Radoslaw Igor
Loh, Han Ping
Chew, Marvin
Hoo, Regina
Baumgarten, Sebastian
Renia, Laurent
Chen, Jianzhu
Preiser, Peter R
Date Issued
2020
Journal
Communications Biology
Publisher
Springer Science and Business Media LLC
Version
Final published version
Abstract
© 2020, The Author(s). The genomes of Plasmodium spp. encode a number of different multigene families that are thought to play a critical role for survival. However, with the exception of the P. falciparum var genes, very little is known about the biological roles of any of the other multigene families. Using the recently developed Selection Linked Integration method, we have been able to activate the expression of a single member of a multigene family of our choice in Plasmodium spp. from its endogenous promoter. We demonstrate the usefulness of this approach by activating the expression of a unique var, rifin and stevor in P. falciparum as well as yir in P. yoelii. Characterization of the selected parasites reveals differences between the different families in terms of mutual exclusive control, co-regulation, and host adaptation. Our results further support the application of the approach for the study of multigene families in Plasmodium and other organisms.
MIT Department
Singapore-MIT Alliance in Research and Technology (SMART)
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/S42003-020-1081-3
