Intracellular Delivery of siRNA by Polycationic Superparamagnetic Nanoparticles
Name
JDD.2012.218940.pdf
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1.39 MB
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Checksum (MD5)
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Author(s)
Castillo, Betzaida
Badillo, Valerie
Hatton, T. Alan
Barletta, Gabriel
Bromberg, Lev E.
Lopez, Xaira
Gonzalez Feliciano, Jose A.
Gonzalez, Carlos I.
Date Issued
2012
Journal
Journal of Drug Delivery
Publisher
Hindawi Publishing Corporation
Citation
Castillo, Betzaida, Lev Bromberg, Xaira López, et al. Intracellular Delivery of siRNA by Polycationic Superparamagnetic Nanoparticles. Journal of Drug Delivery 2012: 1–12. 2012.
Version
Final published version
Abstract
The siRNA transfection efficiency of nanoparticles (NPs), composed of a superparamagnetic iron oxide core modified with polycationic polymers (poly(hexamethylene biguanide) or branched polyethyleneimine), were studied in CHO-K1 and HeLa cell lines. Both NPs demonstrated to be good siRNA transfection vehicles, but unmodified branched polyethyleneimine (25 kD) was superior on both cell lines. However, application of an external magnetic field during transfection (magnetofection) increased the efficiency of the superparamagnetic NPs. Furthermore, our results reveal that these NPs are less toxic towards CHO-K1 cell lines than the unmodified polycationic-branched polyethyleneimine (PEI). In general, the external magnetic field did not alter the cell’s viability nor it disrupted the cell membranes, except for the poly(hexamethylene biguanide)-modified NP, where it was observed that in CHO-K1 cells application of the external magnetic field promoted membrane damage. This paper presents new polycationic superparamagnetic NPs as promising transfection vehicles for siRNA and demonstrates the advantages of magnetofection.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1155/2012/218940
