Characterizing Cellular Biophysical Responses to Stress by Relating Density, Deformability, and Size
Name
byun_biophys-Manilis.pdf
Size
4 MB
Format
Adobe PDF
Checksum (MD5)
94c583fde72b49601ee88824657bbce2
Author(s)
Byun, Sangwon
Hecht, Vivian Chaya
Manalis, Scott R
Date Issued
October 2015
Journal
Biophysical Journal
Publisher
Elsevier
Citation
Byun, Sangwon, Vivian C. Hecht, and Scott R. Manalis. “Characterizing Cellular Biophysical Responses to Stress by Relating Density, Deformability, and Size.” Biophysical Journal 109, no. 8 (October 2015): 1565–1573.
Version
Author's final manuscript
Abstract
Cellular physical properties are important indicators of specific cell states. Although changes in individual biophysical parameters, such as cell size, density, and deformability, during cellular processes have been investigated in great detail, relatively little is known about how they are related. Here, we use a suspended microchannel resonator (SMR) to measure single-cell density, volume, and passage time through a narrow constriction of populations of cells subjected to a variety of environmental stresses. Osmotic stress significantly affects density and volume, as previously shown. In contrast to density and volume, the effect of an osmotic challenge on passage time is relatively small. Deformability, as determined by comparing passage times for cells with similar volume, exhibits a strong dependence on osmolarity, indicating that passage time alone does not always provide a meaningful proxy for deformability. Finally, we find that protein synthesis inhibition, cell-cycle arrest, protein kinase inhibition, and cytoskeletal disruption result in unexpected relationships among deformability, density, and volume. Taken together, our results suggest that by measuring multiple biophysical parameters, one can detect unique characteristics that more specifically reflect cellular behaviors.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.bpj.2015.08.038
