Stable, small, specific, low-valency quantum dots for single-molecule imaging
Name
nihms943934.pdf
Description
Accepted version
Size
521.74 KB
Format
Adobe PDF
Checksum (MD5)
5b202d1453a1260717546680d345a47a
Author(s)
Lee, Jungmin
Feng, Xinyi
Chen, Ou
Bawendi, Moungi G
Huang, Jun
Date Issued
January 2018
Journal
Nanoscale
Publisher
Royal Society of Chemistry (RSC)
Citation
Lee, Jungmin et al. "Stable, small, specific, low-valency quantum dots for single-molecule imaging." Nanoscale 10, 9 (January 2018): 4406-4414
Version
Author's final manuscript
Abstract
We have developed a strategy for synthesizing immediately activable, water-soluble, compact (∼10–12 nm hydrodynamic diameter) quantum dots with a small number of stable and controllable conjugation handles for long distance delivery and subsequent biomolecule conjugation. Upon covalent conjugation with engineered monovalent streptavidin, the sample results in a population consisting of low-valency quantum dots. Alternatively, we have synthesized quantum dots with a small number of biotin molecules that can self-assemble with engineered divalent streptavidin via high-affinity biotin–streptavidin interactions. Being compact, stable and highly specific against biotinylated proteins of interest, these low-valency quantum dots are ideal for labeling and tracking single molecules on the cell surface with high spatiotemporal resolution for different biological systems and applications.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/c7nr08673c
