Cell and fluid sampling microneedle patches for monitoring skin-resident immunity
Name
Combined Revised Manuscript and Supplementary Materials - Clean Copy.pdf
Description
Accepted version
Size
1.63 MB
Format
Adobe PDF
Checksum (MD5)
eaa83f9fcc89fed0084eb7e37170b959
Author(s)
Mandal, Anasuya
Boopathy, Archana V
Lam, Lionel K.W.(Lionel Kar Wei)
Moynihan, Kelly Dare
Welch, Mary E.
Bennett, Nitasha R.
Turvey, Michelle E.
Thai, Nikki Tessa
Van, Jenny H.
Love, Christopher J.
Date Issued
November 2018
Journal
Science Translational Medicine
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Mandal, Anasuya et al. "Cell and fluid sampling microneedle patches for monitoring skin-resident immunity." Science Translational Medicine 10, 467 (November 2018): eaar2227 © 2018 The Authors
Version
Author's final manuscript
Abstract
Important cell populations reside within tissues and are not accessed by traditional blood draws used to monitor the immune system. To address this issue at an essential barrier tissue, the skin, we created a microneedle-based technology for longitudinal sampling of cells and interstitial fluid, enabling minimally invasive parallel monitoring of immune responses. Solid microneedle projections were coated by a cross-linked biocompatible polymer, which swells upon skin insertion, forming a porous matrix for local leukocyte infiltration. By embedding molecular adjuvants and specific antigens encapsulated in nanocapsules within the hydrogel coating, antigen-specific lymphocytes can be enriched in the recovered cell population, allowing for subsequent detailed phenotypic and functional analysis. We demonstrate this approach in mice immunized with a model protein antigen or infected in the skin with vaccinia virus. After vaccination or infection, sampling microneedles allowed tissue-resident memory T cells (TRMs) to be longitudinally monitored in the skin for many months, during which time the antigen-specific T cell population in systemic circulation contracted to low or undetectable counts. Sampling microneedles did not change the immune status of naïve or antigen-exposed animals. We also validated the ability of cell sampling using human skin samples. This approach may be useful in vaccines and immunotherapies to temporally query TRM populations or as a diagnostic platform to sample for biomarkers in chronic inflammatory and autoimmune disorders, allowing information previously accessible only via invasive biopsies to be obtained in a minimally invasive manner from the skin or other mucosal tissues.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/scitranslmed.aar2227
