Principles of Long Noncoding RNA Evolution Derived from Direct Comparison of Transcriptomes in 17 Species
Name
Hezroni-2015-Principles of Long N.pdf
Size
2.27 MB
Format
Adobe PDF
Checksum (MD5)
58337fa8ce0b74b7128228d18751f429
Author(s)
Hezroni, Hadas
Koppstein, David
Schwartz, Matthew G.
Avrutin, Alexandra
Ulitsky, Igor
Bartel, David
Date Issued
May 2015
Journal
Cell Reports
Publisher
Elsevier
Citation
Hezroni, Hadas, David Koppstein, Matthew G. Schwartz, Alexandra Avrutin, David P. Bartel, and Igor Ulitsky. “Principles of Long Noncoding RNA Evolution Derived from Direct Comparison of Transcriptomes in 17 Species.” Cell Reports 11, no. 7 (May 2015): 1110–1122.
Version
Final published version
Abstract
The inability to predict long noncoding RNAs from genomic sequence has impeded the use of comparative genomics for studying their biology. Here, we develop methods that use RNA sequencing (RNA-seq) data to annotate the transcriptomes of 16 vertebrates and the echinoid sea urchin, uncovering thousands of previously unannotated genes, most of which produce long intervening noncoding RNAs (lincRNAs). Although in each species, >70% of lincRNAs cannot be traced to homologs in species that diverged >50 million years ago, thousands of human lincRNAs have homologs with similar expression patterns in other species. These homologs share short, 5′-biased patches of sequence conservation nested in exonic architectures that have been extensively rewired, in part by transposable element exonization. Thus, over a thousand human lincRNAs are likely to have conserved functions in mammals, and hundreds beyond mammals, but those functions require only short patches of specific sequences and can tolerate major changes in gene architecture.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.celrep.2015.04.023
