Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation
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Author(s)
Davies, Bryan W.
Koehrer, Caroline
Walker, Graham C.
Jacob, Asha I
Rajbhandary, Uttam L
Date Issued
December 2012
Journal
Molecular Cell
Publisher
Elsevier
Citation
Jacob, Asha Ivy, Caroline Kohrer, Bryan William Davies, Uttam Lal RajBhandary, and Graham Charles Walker. “Conserved Bacterial RNase YbeY Plays Key Roles in 70S Ribosome Quality Control and 16S rRNA Maturation.” Molecular Cell 49, no. 3 (February 2013): 427–438. © 2013 Elsevier Inc.
Version
Final published version
Abstract
Quality control of ribosomes is critical for cellular function since protein mistranslation leads to severe physiological consequences. We report evidence of a previously unrecognized ribosome quality control system in bacteria that operates at the level of 70S to remove defective ribosomes. YbeY, a previously unidentified endoribonuclease, and the exonuclease RNase R act together by a process mediated specifically by the 30S ribosomal subunit, to degrade defective 70S ribosomes but not properly matured 70S ribosomes or individual subunits. Furthermore, there is essentially no fully matured 16S rRNA in a ΔybeY mutant at 45°C, making YbeY the only endoribonuclease to be implicated in the critically important processing of the 16S rRNA 3′ terminus. These key roles in ribosome quality control and maturation indicate why YbeY is a member of the minimal bacterial gene set and suggest that it could be a potential target for antibacterial drugs.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1016/j.molcel.2012.11.025
