Target specificity of the CRISPR-Cas9 system
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Sharp_Target specificity.pdf
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305.59 KB
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Author(s)
Wu, Xuebing
Kriz, Andrea J.
Sharp, Phillip A.
Date Issued
August 2014
Journal
Quantitative Biology
Publisher
Springer-Verlag
Citation
Wu, Xuebing, Andrea J. Kriz, and Phillip A. Sharp. “Target Specificity of the CRISPR-Cas9 System.” Quantitative Biology 2, no. 2 (June 2014): 59–70.
Version
Author's final manuscript
Abstract
The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1007/s40484-014-0030-x
