Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure
Name
27554-1057574-1-PB.pdf
Description
Published version
Size
1.81 MB
Format
Unknown
Checksum (MD5)
4cd06cdc1f2d5ace481dbe3f22f81e9a
Author(s)
Vasuri, Francesco
Degiovanni, Alessio
Gargiulo, Mauro
Thilly, William G
Gostjeva, Elena V.
Pasquinelli, Gianandrea
Fittipaldi, Silvia
Date Issued
2020
Journal
Oncotarget
Publisher
Impact Journals, LLC
Version
Final published version
Abstract
Copyright: © Vasuri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. We evaluated the long-term effects of sirolimus on three different cell in vitro models, cultured in physiological conditions mimicking sirolimus-eluted stent, in order to clarify the effectiveness of sirolimus in blocking cell proliferation and survival. Three cells lines (WPMY-1 myofibroblasts, HT-29 colorectal adenocarcinoma, and U2OS osteosarcoma) were selected and growth in 10 ml of Minimum Essential Medium for 5 weeks with serial dilutions of sirolimus. The number of colonies and the number of cells per colony were counted. As main result, the number of WPMY-1 surviving colonies increased in a dose-dependent manner when treated with sirolimus (p = 0.0011), while the number of U2OS colonies progressively decreased (p = 0.0011). The clonal capacity of HT-29 was not modified by the exposure to sirolimus (p = 0.6679). In conclusion sirolimus showed the well-known cytostatic effect, but with an effect on clonogenic potential different among the different cell types. In the practice, the plaque typology and composition may influence the response to sirolimus and thus the effectiveness of eluted stent.
MIT Department
Massachusetts Institute of Technology. Laboratory in Metakaryotic Biology
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 3.0 unported license
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DOI of Published Version
https://doi.org/10.18632/ONCOTARGET.27554
