Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging
Name
Giacomelli-2016-Virtual Hematoxylin.pdf
Size
5.38 MB
Format
Adobe PDF
Checksum (MD5)
d85987e485c80a1c36909334b4db24e8
Author(s)
Husvogt, Lennart
Vardeh, Hilde
Faulkner-Jones, Beverly E.
Hornegger, Joachim
Connolly, James L.
Giacomelli, Michael
Fujimoto, James G
Date Issued
August 2016
Journal
PLOS ONE
Publisher
Public Library of Science
Citation
Giacomelli, Michael G. et al. “Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging.” Ed. Thomas Abraham. PLOS ONE 11.8 (2016): e0159337.
Version
Final published version
Abstract
We derive a physically realistic model for the generation of virtual transillumination, white light microscopy images using epi-fluorescence measurements from thick, unsectioned tissue. We demonstrate this technique by generating virtual transillumination H&E images of unsectioned human breast tissue from epi-fluorescence multiphoton microscopy data. The virtual transillumination algorithm is shown to enable improved contrast and color accuracy compared with previous color mapping methods. Finally, we present an open source implementation of the algorithm in OpenGL, enabling real-time GPU-based generation of virtual transillumination microscopy images using conventional fluorescence microscopy systems.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1371/journal.pone.0159337
