Single-Cell Genomics Reveals Hundreds of Coexisting Subpopulations in Wild Prochlorococcus
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Kashtan_etal_Science2014.pdf
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Author(s)
Kashtan, Nadav
Roggen, Sara E.
Rodrigue, Sebastien
Thompson, Jessica Weidemier
Biller, Steven
Coe, Allison
Ding, Huiming
Marttinen, Pekka
Malmstrom, Rex R.
Stocker, Roman
Date Issued
April 2014
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Kashtan, N., S. E. Roggensack, S. Rodrigue, J. W. Thompson, S. J. Biller, A. Coe, H. Ding, et al. “Single-Cell Genomics Reveals Hundreds of Coexisting Subpopulations in Wild Prochlorococcus.” Science 344, no. 6182 (April 24, 2014): 416–420.
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Author's final manuscript
Abstract
Extensive genomic diversity within coexisting members of a microbial species has been revealed through selected cultured isolates and metagenomic assemblies. Yet, the cell-by-cell genomic composition of wild uncultured populations of co-occurring cells is largely unknown. In this work, we applied large-scale single-cell genomics to study populations of the globally abundant marine cyanobacterium Prochlorococcus. We show that they are composed of hundreds of subpopulations with distinct “genomic backbones,” each backbone consisting of a different set of core gene alleles linked to a small distinctive set of flexible genes. These subpopulations are estimated to have diverged at least a few million years ago, suggesting ancient, stable niche partitioning. Such a large set of coexisting subpopulations may be a general feature of free-living bacterial species with huge populations in highly mixed habitats.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.1248575
