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Chromosome organization by one-sided and two-sided loop extrusion
Name
elife-53558-v2.pdf
Description
Published version
Size
4.39 MB
Format
Adobe PDF
Checksum (MD5)
eafafd623e2335471d3414265dcb8685
Author(s)
Banigan, Edward J
van den Berg, Aafke A
Brandão, Hugo B
Marko, John F
Mirny, Leonid A
Date Issued
2020
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Banigan, Edward J, van den Berg, Aafke A, Brandão, Hugo B, Marko, John F and Mirny, Leonid A. 2020. "Chromosome organization by one-sided and two-sided loop extrusion." eLife, 9.
Version
Final published version
Abstract
© 2020, eLife Sciences Publications Ltd. All rights reserved. SMC complexes, such as condensin or cohesin, organize chromatin throughout the cell cycle by a process known as loop extrusion. SMC complexes reel in DNA, extruding and progressively growing DNA loops. Modeling assuming two-sided loop extrusion reproduces key features of chromatin organization across different organisms. In vitro single-molecule experiments confirmed that yeast condensins extrude loops, however, they remain anchored to their loading sites and extrude loops in a “one-sided” manner. We therefore simulate one-sided loop extrusion to investigate whether “one-sided” complexes can compact mitotic chromosomes, organize interphase domains, and juxtapose bacterial chromosomal arms, as can be done by “two-sided” loop extruders. While one-sided loop extrusion cannot reproduce these phenomena, variants can recapitulate in vivo observations. We predict that SMC complexes in vivo constitute effectively two-sided motors or exhibit biased loading and propose relevant experiments. Our work suggests that loop extrusion is a viable general mechanism of chromatin organization.
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
10.7554/ELIFE.53558
