Comparison of Cas9 activators in multiple species
Name
Collins_Comparison of cas9.pdf
Size
1.04 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Chavez, Alejandro
Tuttle, Marcelle
Pruitt, Benjamin W
Ewen-Campen, Ben
Chari, Raj
Ter-Ovanesyan, Dmitry
Haque, Sabina J
Cecchi, Ryan J
Kowal, Emma J K
Buchthal, Joanna
Date Issued
May 2016
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
.Chavez, Alejandro et al. “Comparison of Cas9 Activators in Multiple Species.” Nature Methods 13.7 (2016): 563–567.
Version
Author's final manuscript
Abstract
Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Synthetic Biology Center
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmeth.3871
